Replacing fetal bovine serum (FBS) in research and testing
نویسندگان
چکیده
منابع مشابه
Fetal Bovine Serum (FBS): Past - Present - Future.
The supplementation of culture medium with fetal bovine serum (FBS, also referred to as "fetal calf serum") is still common practice in cell culture applications. Due to a number of disadvantages in terms of quality and reproducibility of in vitro data, animal welfare concerns, and in light of recent cases of fraudulent marketing, the search for alternatives and the development of serum-free me...
متن کاملConsensus Report Fetal Bovine Serum (FBS): Past – Present – Future
Jan van der Valk, Karen Bieback, Christiane Buta, Brett Cochrane Wilhelm G. Dirks, Jianan Fu, James J. Hickman, Christiane Hohensee, Roman Kolar, Manfred Liebsch, Francesca Pistollato, Markus Schulz, Daniel Thieme, Tilo Weber, Joachim Wiest, Stefan Winkler and Gerhard Gstraunthaler The 3Rs-Centre Utrecht Life Sciences, Utrecht University, Utrecht, The Netherlands; Institute of Transfusion Medic...
متن کاملPlatelet Rich in Growth Factors (PRGF): A Suitable Replacement for Fetal Bovine Serum (FBS) in Mesenchymal Stem Cell Culture
Background: Due to high differentiation potential and self-renewality, Mesenchymal Stem Cells are now widely considered by researchers in several diseases. FBS is used as a supplement in culture media for proliferation, differentiation, and other culture processes of MSCs, which is associated with transmission risk of a variety of infections as well as immune responses. PRGF derived from platel...
متن کاملGoat serum: an alternative to fetal bovine serum in biomedical research.
Serum is frequently added to the defined basal medium as a source of certain nutritional and macromolecular growth factors essential for cell growth. Although a number of synthetic media have been prepared serum continues to be used in cell culture by many investigators. The best supplementation to a basal medium is fetal bovine serum (FBS) that is most frequently used for all types of cell cul...
متن کاملResearch Article: In vitro investigation on response of cultivated hepatocytes from Klunzinger\'s mullet, Liza klunzingeri, to temperature and fetal bovine serum
The appropriate temperature and growth supplementary factors such as fetal bovine serum (FBS) are the necessities needed for cell growth in culture medium. In this regards, the effect of Leibovitz-15 (L-15) medium containing different concentrations of fetal bovine serum (FBS) was assessed on growth and proliferation of cultivated liver cells of klunzingerchr('39')s mullet, Liza klunzingeri, in...
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ژورنال
عنوان ژورنال: Alternatives to animal experimentation
سال: 2021
ISSN: ['1868-596X', '1868-8551']
DOI: https://doi.org/10.14573/altex.2012141